mouse anti pstat1 Search Results


rabbit anti pstat1  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc rabbit anti pstat1
Rabbit Anti Pstat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+pstat1/Phospho-Stat1+(Tyr701)+Rabbit+mAb/pmc12031792-61-78-83
Average 97 stars, based on 1 article reviews
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rabbit anti pstat1  (R&D Systems)
94
R&D Systems rabbit anti pstat1

Rabbit Anti Pstat1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+pstat1/Human%2FMouse+Phospho-STAT1+(Y701)+Antibody/pmc10214391-10-0-3
Average 94 stars, based on 1 article reviews
rabbit anti pstat1 - by Bioz Stars, 2026-09
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rabbit anti pstat1 igg  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit anti pstat1 igg

Rabbit Anti Pstat1 Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+pstat1/Phospho-Stat1+(Ser727)+Rabbit+mAb/10__1089_slash_aid__2018__0132-108-15-21
Average 95 stars, based on 1 article reviews
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cleaved caspase 3  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc cleaved caspase 3

Cleaved Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+pstat1/Caspase-3+Antibody/pm37600826-52-26-32
Average 99 stars, based on 1 article reviews
cleaved caspase 3 - by Bioz Stars, 2026-09
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anti pstat1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti pstat1

Anti Pstat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+pstat1/Phospho-Stat1+(Ser727)+Antibody/pmc08018097-222-53-56
Average 96 stars, based on 1 article reviews
anti pstat1 - by Bioz Stars, 2026-09
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monoclonal mouse anti-pstat1(y701  (Becton Dickinson)
90
Becton Dickinson monoclonal mouse anti-pstat1(y701
SeV-induced IFN production activates Jak/STAT signaling and ISG induction. (A) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. Nuclear and cytoplasmic protein extracts were prepared and western blots probing for pIRF-3(S386), IRF-3, IRF-7, and IRF-9 were performed. RNA pol II and β-tubulin were the nuclear and cytoplasmic loading controls, respectively. N, nuclear, C, cytoplasmic. (B) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole cell protein lysates were prepared. Western blots probing for <t>pSTAT1(Y701),</t> STAT1(total), pSTAT2(Y689), STAT2(total), ISG15, and IFIT3, and tubulin as the loading control were performed. (C) A549 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole protein lysates were prepared. Western blots probing for pSTAT1(Y701), STAT1(total), pSTAT2(Y689), STAT2(total), IRF-9, IRF-7, and Mx1, and tubulin as the loading control were performed. (D) ISG15 and IFIT3 mRNA expression in U937 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. (E) IRF-7 and Mx1 mRNA expression in A549 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. IRF-7 and Mx1 mRNA induction in (F) primary monocytes and (G) Namalwa cells infected with SeV (150 HA U/mL) for 24 h. All gene induction data are expressed as the % of relative HPRT expression. All data are representative of 3 independent biological replicates. UI, uninfected, untreated. IRF, IFN regulatory factor; ISG, interferon-stimulated gene; HPRT, hypoxanthine-guanine phosphoribosyltransferase.
Monoclonal Mouse Anti Pstat1(y701, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+pstat1/cytofix+cytoperm+fixation+permeabilization+kit/pmc05105340-215-43-46
Average 90 stars, based on 1 article reviews
monoclonal mouse anti-pstat1(y701 - by Bioz Stars, 2026-09
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anti phospho stat1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti phospho stat1
SeV-induced IFN production activates Jak/STAT signaling and ISG induction. (A) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. Nuclear and cytoplasmic protein extracts were prepared and western blots probing for pIRF-3(S386), IRF-3, IRF-7, and IRF-9 were performed. RNA pol II and β-tubulin were the nuclear and cytoplasmic loading controls, respectively. N, nuclear, C, cytoplasmic. (B) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole cell protein lysates were prepared. Western blots probing for <t>pSTAT1(Y701),</t> STAT1(total), pSTAT2(Y689), STAT2(total), ISG15, and IFIT3, and tubulin as the loading control were performed. (C) A549 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole protein lysates were prepared. Western blots probing for pSTAT1(Y701), STAT1(total), pSTAT2(Y689), STAT2(total), IRF-9, IRF-7, and Mx1, and tubulin as the loading control were performed. (D) ISG15 and IFIT3 mRNA expression in U937 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. (E) IRF-7 and Mx1 mRNA expression in A549 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. IRF-7 and Mx1 mRNA induction in (F) primary monocytes and (G) Namalwa cells infected with SeV (150 HA U/mL) for 24 h. All gene induction data are expressed as the % of relative HPRT expression. All data are representative of 3 independent biological replicates. UI, uninfected, untreated. IRF, IFN regulatory factor; ISG, interferon-stimulated gene; HPRT, hypoxanthine-guanine phosphoribosyltransferase.
Anti Phospho Stat1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+pstat1/Phospho-Stat1+(Tyr701)+Rabbit+mAb/pmc04546911-146-5-21
Average 96 stars, based on 1 article reviews
anti phospho stat1 - by Bioz Stars, 2026-09
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anti pstat1  (Danaher Inc)
99
Danaher Inc anti pstat1
SeV-induced IFN production activates Jak/STAT signaling and ISG induction. (A) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. Nuclear and cytoplasmic protein extracts were prepared and western blots probing for pIRF-3(S386), IRF-3, IRF-7, and IRF-9 were performed. RNA pol II and β-tubulin were the nuclear and cytoplasmic loading controls, respectively. N, nuclear, C, cytoplasmic. (B) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole cell protein lysates were prepared. Western blots probing for <t>pSTAT1(Y701),</t> STAT1(total), pSTAT2(Y689), STAT2(total), ISG15, and IFIT3, and tubulin as the loading control were performed. (C) A549 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole protein lysates were prepared. Western blots probing for pSTAT1(Y701), STAT1(total), pSTAT2(Y689), STAT2(total), IRF-9, IRF-7, and Mx1, and tubulin as the loading control were performed. (D) ISG15 and IFIT3 mRNA expression in U937 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. (E) IRF-7 and Mx1 mRNA expression in A549 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. IRF-7 and Mx1 mRNA induction in (F) primary monocytes and (G) Namalwa cells infected with SeV (150 HA U/mL) for 24 h. All gene induction data are expressed as the % of relative HPRT expression. All data are representative of 3 independent biological replicates. UI, uninfected, untreated. IRF, IFN regulatory factor; ISG, interferon-stimulated gene; HPRT, hypoxanthine-guanine phosphoribosyltransferase.
Anti Pstat1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+pstat1/Recombinant+Anti-Human+IgG+antibody/pmc04959311-141-56-77
Average 99 stars, based on 1 article reviews
anti pstat1 - by Bioz Stars, 2026-09
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mouse anti pstat1  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology mouse anti pstat1
SeV-induced IFN production activates Jak/STAT signaling and ISG induction. (A) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. Nuclear and cytoplasmic protein extracts were prepared and western blots probing for pIRF-3(S386), IRF-3, IRF-7, and IRF-9 were performed. RNA pol II and β-tubulin were the nuclear and cytoplasmic loading controls, respectively. N, nuclear, C, cytoplasmic. (B) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole cell protein lysates were prepared. Western blots probing for <t>pSTAT1(Y701),</t> STAT1(total), pSTAT2(Y689), STAT2(total), ISG15, and IFIT3, and tubulin as the loading control were performed. (C) A549 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole protein lysates were prepared. Western blots probing for pSTAT1(Y701), STAT1(total), pSTAT2(Y689), STAT2(total), IRF-9, IRF-7, and Mx1, and tubulin as the loading control were performed. (D) ISG15 and IFIT3 mRNA expression in U937 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. (E) IRF-7 and Mx1 mRNA expression in A549 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. IRF-7 and Mx1 mRNA induction in (F) primary monocytes and (G) Namalwa cells infected with SeV (150 HA U/mL) for 24 h. All gene induction data are expressed as the % of relative HPRT expression. All data are representative of 3 independent biological replicates. UI, uninfected, untreated. IRF, IFN regulatory factor; ISG, interferon-stimulated gene; HPRT, hypoxanthine-guanine phosphoribosyltransferase.
Mouse Anti Pstat1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+pstat1/p-Stat1+Antibody/pmc12052582-520-14-16
Average 94 stars, based on 1 article reviews
mouse anti pstat1 - by Bioz Stars, 2026-09
94/100 stars
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gene exp lgals8 hs01057135 m1  (Thermo Fisher)
85
Thermo Fisher gene exp lgals8 hs01057135 m1
SeV-induced IFN production activates Jak/STAT signaling and ISG induction. (A) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. Nuclear and cytoplasmic protein extracts were prepared and western blots probing for pIRF-3(S386), IRF-3, IRF-7, and IRF-9 were performed. RNA pol II and β-tubulin were the nuclear and cytoplasmic loading controls, respectively. N, nuclear, C, cytoplasmic. (B) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole cell protein lysates were prepared. Western blots probing for <t>pSTAT1(Y701),</t> STAT1(total), pSTAT2(Y689), STAT2(total), ISG15, and IFIT3, and tubulin as the loading control were performed. (C) A549 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole protein lysates were prepared. Western blots probing for pSTAT1(Y701), STAT1(total), pSTAT2(Y689), STAT2(total), IRF-9, IRF-7, and Mx1, and tubulin as the loading control were performed. (D) ISG15 and IFIT3 mRNA expression in U937 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. (E) IRF-7 and Mx1 mRNA expression in A549 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. IRF-7 and Mx1 mRNA induction in (F) primary monocytes and (G) Namalwa cells infected with SeV (150 HA U/mL) for 24 h. All gene induction data are expressed as the % of relative HPRT expression. All data are representative of 3 independent biological replicates. UI, uninfected, untreated. IRF, IFN regulatory factor; ISG, interferon-stimulated gene; HPRT, hypoxanthine-guanine phosphoribosyltransferase.
Gene Exp Lgals8 Hs01057135 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+pstat1/Gene+Exp%2E+LGALS8%2C+Hs01057135_m1/us12599592-725-101-99
Average 85 stars, based on 1 article reviews
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Image Search Results


Journal: iScience

Article Title: C9ORF72 suppresses JAK-STAT mediated inflammation

doi: 10.1016/j.isci.2023.106579

Figure Lengend Snippet:

Article Snippet: rabbit anti-pSTAT1 , R and D Systems , Cat# AF2894, RRID: AB_2198137.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Bradford Protein Assay, CRISPR, Plasmid Preparation, Software

SeV-induced IFN production activates Jak/STAT signaling and ISG induction. (A) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. Nuclear and cytoplasmic protein extracts were prepared and western blots probing for pIRF-3(S386), IRF-3, IRF-7, and IRF-9 were performed. RNA pol II and β-tubulin were the nuclear and cytoplasmic loading controls, respectively. N, nuclear, C, cytoplasmic. (B) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole cell protein lysates were prepared. Western blots probing for pSTAT1(Y701), STAT1(total), pSTAT2(Y689), STAT2(total), ISG15, and IFIT3, and tubulin as the loading control were performed. (C) A549 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole protein lysates were prepared. Western blots probing for pSTAT1(Y701), STAT1(total), pSTAT2(Y689), STAT2(total), IRF-9, IRF-7, and Mx1, and tubulin as the loading control were performed. (D) ISG15 and IFIT3 mRNA expression in U937 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. (E) IRF-7 and Mx1 mRNA expression in A549 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. IRF-7 and Mx1 mRNA induction in (F) primary monocytes and (G) Namalwa cells infected with SeV (150 HA U/mL) for 24 h. All gene induction data are expressed as the % of relative HPRT expression. All data are representative of 3 independent biological replicates. UI, uninfected, untreated. IRF, IFN regulatory factor; ISG, interferon-stimulated gene; HPRT, hypoxanthine-guanine phosphoribosyltransferase.

Journal: Journal of Interferon & Cytokine Research

Article Title: Type I Interferon-Mediated Induction of Antiviral Genes and Proteins Fails to Protect Cells from the Cytopathic Effects of Sendai Virus Infection

doi: 10.1089/jir.2016.0051

Figure Lengend Snippet: SeV-induced IFN production activates Jak/STAT signaling and ISG induction. (A) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. Nuclear and cytoplasmic protein extracts were prepared and western blots probing for pIRF-3(S386), IRF-3, IRF-7, and IRF-9 were performed. RNA pol II and β-tubulin were the nuclear and cytoplasmic loading controls, respectively. N, nuclear, C, cytoplasmic. (B) U937 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole cell protein lysates were prepared. Western blots probing for pSTAT1(Y701), STAT1(total), pSTAT2(Y689), STAT2(total), ISG15, and IFIT3, and tubulin as the loading control were performed. (C) A549 cells were infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h and whole protein lysates were prepared. Western blots probing for pSTAT1(Y701), STAT1(total), pSTAT2(Y689), STAT2(total), IRF-9, IRF-7, and Mx1, and tubulin as the loading control were performed. (D) ISG15 and IFIT3 mRNA expression in U937 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. (E) IRF-7 and Mx1 mRNA expression in A549 cells infected with SeV (150 HA U/mL) for 4, 8, 16, and 24 h. IRF-7 and Mx1 mRNA induction in (F) primary monocytes and (G) Namalwa cells infected with SeV (150 HA U/mL) for 24 h. All gene induction data are expressed as the % of relative HPRT expression. All data are representative of 3 independent biological replicates. UI, uninfected, untreated. IRF, IFN regulatory factor; ISG, interferon-stimulated gene; HPRT, hypoxanthine-guanine phosphoribosyltransferase.

Article Snippet: The following antibodies and dilutions were used for the western blot analyses: Monoclonal rabbit anti-pIRF-3(S386; Abcam, Cambridge, MA): 1:5,000; monoclonal anti-IRF-3 (Cell Signaling Technologies, Danvers, MA): 1:1,000; polyclonal rabbit anti-IRF-7 (Santa Cruz Biotechnologies, Dallas, TX): 1:1,000; polyclonal rabbit anti-STAT1 (Santa Cruz Biotechnologies): 1:1,000; monoclonal mouse anti-pSTAT1(Y701; BD Transduction Laboratories, San Jose, CA): 1:5,000; polyclonal rabbit anti-STAT2 (Santa Cruz Biotechnologies): 1:1,000; polyclonal rabbit anti-pSTAT2(Y689; End Millipore, Billerica, MA): 1:2,000; monoclonal mouse anti-IRF-9(ISGF3γ; BD Transduction Laboratories): 1:1,000; monoclonal mouse anti-Actin (Cell Signaling Technologies): 1:5,000; polyclonal rabbit anti-β tubulin (Cell Signaling Technologies): 1:2,000; mouse monoclonal RNA polymerase II (Santa Cruz Biotechnologies): 1:1,000; rabbit polyclonal IFIT3 (Covance, Inc., Denver, PA) 1:1,000; rabbit polyclonal ISG15: 1:1,000 (Santa Cruz Biotechnologies); mouse polyclonal Mx1: 1:1,000 (Abnova, Taipei, Taiwan).

Techniques: Infection, Western Blot, Expressing

Inhibiting canonical type I IFN signaling during SeV infection does not affect SeV-mediated CPE. (A) U937 cells were treated with 1000 U/mL of IFN-α2a, IFN-β, or IFN-ω for 1 h in the presence or absence of the IFNAR2 neutralizing antibody. Western blot analysis was performed to detect phosphorylated STAT1(Y701) and tubulin was used as the loading control. UT: untreated; NA: cells treated with IFNAR2 neutralizing antibody alone; α2a: IFN-α2a treated; α2a + NA: cells treated with both antibody and IFN-α2a; β: IFN-β treated; β + NA: cells treated with both antibody and IFN-β; ω: IFN-ω treated; and ω + NA: cells treated with both antibody and IFN-ω. (B) U937 (panel 1) and A549 cells (panel 2) were infected with SeV (150 HA U/mL) in the presence or absence of IFNAR2 neutralizing antibody. Cell viability was measured 72 h pi. Data are representative of 3 independent biological replicates. Supernatants from cells infected with SeV (150 HA U/mL) for 16 h were inactivated (β-propiolactone) and used to pretreat fresh (C) U937 and (D) A549 cells for 24 h in the presence or absence of the IFNAR or IFNLR neutralizing antibodies. The U937 cells were then infected with either EMCV or SeV (150 HA U/mL) for 72 h and cell viability was measured via MTT assay (U937) or crystal violet assay (A549). Results are shown as the % of cell control. SeV150: virus-inactivated supernatant from SeV-infected cells (150 HA U/mL); SeV150 + IFNAR2 NA: treated with virus-inactivated supernatant from SeV-infected cells (150 HA U/mL) in the presence of IFNAR2-neutralizing antibody; SeV150 + IFNLR NA: treated with virus-inactivated supernatant from SeV-infected cells (150 HA U/mL) in the presence of IFNLR-neutralizing antibody. IFNAR, IFN receptor; IFNLR, IFN-lambda receptor.

Journal: Journal of Interferon & Cytokine Research

Article Title: Type I Interferon-Mediated Induction of Antiviral Genes and Proteins Fails to Protect Cells from the Cytopathic Effects of Sendai Virus Infection

doi: 10.1089/jir.2016.0051

Figure Lengend Snippet: Inhibiting canonical type I IFN signaling during SeV infection does not affect SeV-mediated CPE. (A) U937 cells were treated with 1000 U/mL of IFN-α2a, IFN-β, or IFN-ω for 1 h in the presence or absence of the IFNAR2 neutralizing antibody. Western blot analysis was performed to detect phosphorylated STAT1(Y701) and tubulin was used as the loading control. UT: untreated; NA: cells treated with IFNAR2 neutralizing antibody alone; α2a: IFN-α2a treated; α2a + NA: cells treated with both antibody and IFN-α2a; β: IFN-β treated; β + NA: cells treated with both antibody and IFN-β; ω: IFN-ω treated; and ω + NA: cells treated with both antibody and IFN-ω. (B) U937 (panel 1) and A549 cells (panel 2) were infected with SeV (150 HA U/mL) in the presence or absence of IFNAR2 neutralizing antibody. Cell viability was measured 72 h pi. Data are representative of 3 independent biological replicates. Supernatants from cells infected with SeV (150 HA U/mL) for 16 h were inactivated (β-propiolactone) and used to pretreat fresh (C) U937 and (D) A549 cells for 24 h in the presence or absence of the IFNAR or IFNLR neutralizing antibodies. The U937 cells were then infected with either EMCV or SeV (150 HA U/mL) for 72 h and cell viability was measured via MTT assay (U937) or crystal violet assay (A549). Results are shown as the % of cell control. SeV150: virus-inactivated supernatant from SeV-infected cells (150 HA U/mL); SeV150 + IFNAR2 NA: treated with virus-inactivated supernatant from SeV-infected cells (150 HA U/mL) in the presence of IFNAR2-neutralizing antibody; SeV150 + IFNLR NA: treated with virus-inactivated supernatant from SeV-infected cells (150 HA U/mL) in the presence of IFNLR-neutralizing antibody. IFNAR, IFN receptor; IFNLR, IFN-lambda receptor.

Article Snippet: The following antibodies and dilutions were used for the western blot analyses: Monoclonal rabbit anti-pIRF-3(S386; Abcam, Cambridge, MA): 1:5,000; monoclonal anti-IRF-3 (Cell Signaling Technologies, Danvers, MA): 1:1,000; polyclonal rabbit anti-IRF-7 (Santa Cruz Biotechnologies, Dallas, TX): 1:1,000; polyclonal rabbit anti-STAT1 (Santa Cruz Biotechnologies): 1:1,000; monoclonal mouse anti-pSTAT1(Y701; BD Transduction Laboratories, San Jose, CA): 1:5,000; polyclonal rabbit anti-STAT2 (Santa Cruz Biotechnologies): 1:1,000; polyclonal rabbit anti-pSTAT2(Y689; End Millipore, Billerica, MA): 1:2,000; monoclonal mouse anti-IRF-9(ISGF3γ; BD Transduction Laboratories): 1:1,000; monoclonal mouse anti-Actin (Cell Signaling Technologies): 1:5,000; polyclonal rabbit anti-β tubulin (Cell Signaling Technologies): 1:2,000; mouse monoclonal RNA polymerase II (Santa Cruz Biotechnologies): 1:1,000; rabbit polyclonal IFIT3 (Covance, Inc., Denver, PA) 1:1,000; rabbit polyclonal ISG15: 1:1,000 (Santa Cruz Biotechnologies); mouse polyclonal Mx1: 1:1,000 (Abnova, Taipei, Taiwan).

Techniques: Infection, Western Blot, MTT Assay, Crystal Violet Assay